pre mir 497 Search Results


(mmu482607_mir)  (Thermo Fisher)
93
Thermo Fisher (mmu482607_mir)
(Mmu482607 Mir), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirna 1st strand cdna synthesis kit  (Vazyme Biotech Co)
98
Vazyme Biotech Co mirna 1st strand cdna synthesis kit
Mirna 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micrornas primers  (Qiagen)
90
Qiagen micrornas primers
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Micrornas Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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pre-mir-497  (Thermo Fisher)
90
Thermo Fisher pre-mir-497
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Pre Mir 497, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pre mir 497 195  (Addgene inc)
91
Addgene inc human pre mir 497 195
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Human Pre Mir 497 195, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse pre-mir-497~195 cdna  (GenScript corporation)
90
GenScript corporation mouse pre-mir-497~195 cdna
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Mouse Pre Mir 497~195 Cdna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre-mir-497 mi0003138  (Thermo Fisher)
90
Thermo Fisher pre-mir-497 mi0003138
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Pre Mir 497 Mi0003138, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mircury lna mirna mimics mir-497-5p  (Qiagen)
90
Qiagen mircury lna mirna mimics mir-497-5p
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Mircury Lna Mirna Mimics Mir 497 5p, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirvana mirna inhibitors  (Thermo Fisher)
90
Thermo Fisher mirvana mirna inhibitors
CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of <t>microRNAs</t> targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.
Mirvana Mirna Inhibitors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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advanced mirna cfa mir 497 478138 mir  (Thermo Fisher)
91
Thermo Fisher advanced mirna cfa mir 497 478138 mir
The expression of miRNAs in cisplatin-sensitive and -resistant cervical cancer cells. Effects of cisplatin at different concentrations on cell viability were analyzed using CCK-8 assay in SiHa and SiHa/DDP (A) , and in HeLa and HeLa/DDP (B) . (C) Differentially expressed miRNAs were analyzed between cervical cancer tissues and the adjacent normal tissues group. Data were retrieved from Gene Expression Omnibus (GEO) dataset, with the accession number GSE19611. The color code in the heat map is linear and the expression levels of miRNAs that were upregulated are shown in green to red, whereas the miRNAs that were downregulated are shown from red to green. (D, E) miR-92a-3p, miR-221-3p, miR-21, miR-146a, miR-196a, miR-383, miR-206 and <t>miR-497</t> were further analyzed using qRT-PCR in SiHa/DDP and HeLa/DDP cells. Data are presented as means ± SD of three individual experiments. * p < 0.05, ** p < 0.01 vs . SiHa or HeLa cells.
Advanced Mirna Cfa Mir 497 478138 Mir, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microrna mimics (pre-mir-497  (Thermo Fisher)
90
Thermo Fisher microrna mimics (pre-mir-497
The expression of miRNAs in cisplatin-sensitive and -resistant cervical cancer cells. Effects of cisplatin at different concentrations on cell viability were analyzed using CCK-8 assay in SiHa and SiHa/DDP (A) , and in HeLa and HeLa/DDP (B) . (C) Differentially expressed miRNAs were analyzed between cervical cancer tissues and the adjacent normal tissues group. Data were retrieved from Gene Expression Omnibus (GEO) dataset, with the accession number GSE19611. The color code in the heat map is linear and the expression levels of miRNAs that were upregulated are shown in green to red, whereas the miRNAs that were downregulated are shown from red to green. (D, E) miR-92a-3p, miR-221-3p, miR-21, miR-146a, miR-196a, miR-383, miR-206 and <t>miR-497</t> were further analyzed using qRT-PCR in SiHa/DDP and HeLa/DDP cells. Data are presented as means ± SD of three individual experiments. * p < 0.05, ** p < 0.01 vs . SiHa or HeLa cells.
Microrna Mimics (Pre Mir 497, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mirna 3´utr target expression clone for human mthfsd  (Genecopoeia)
N/A
GeneCopoeia offers genome-wide human, mouse and rat microRNA (miRNA) 3′ UTR target clones in mammalian expression vectors. miRNA 3′ UTR target clones can be used for miRNA target identification and functional validation of predicted targets,
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Image Search Results


CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of microRNAs targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.

Journal: Theranostics

Article Title: Cardiomyocyte-derived small extracellular vesicles can signal eNOS activation in cardiac microvascular endothelial cells to protect against Ischemia/Reperfusion injury

doi: 10.7150/thno.43163

Figure Lengend Snippet: CM-derived sEVsmediate eNOSactivation by increasing p70s6k1 in CMECs through reduction of miR-145-5p. A-C) The level of microRNAs targeting the p70s6k1 mRNA in CMECs (n = 4). D-G) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=3). H-I) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs. CM TXL +NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM TXL : TXL-pretreated CMs; CM TXL +Mimic group: CMECs were transfected with miR-145-5p mimics before being co-cultured with CM TXL and then exposed to H/R; CM TXL +NC: CMECs were transfected with negative control of mimics before being co-cultured with CM TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.

Article Snippet: Specific primers for microRNAs (miR-497-5p, miR-145-5p, miR-128-3p, and U6) were purchased from Qiagen.

Techniques: Derivative Assay, Western Blot, Flow Cytometry, Control, Transfection, Cell Culture, Negative Control

The expression of miRNAs in cisplatin-sensitive and -resistant cervical cancer cells. Effects of cisplatin at different concentrations on cell viability were analyzed using CCK-8 assay in SiHa and SiHa/DDP (A) , and in HeLa and HeLa/DDP (B) . (C) Differentially expressed miRNAs were analyzed between cervical cancer tissues and the adjacent normal tissues group. Data were retrieved from Gene Expression Omnibus (GEO) dataset, with the accession number GSE19611. The color code in the heat map is linear and the expression levels of miRNAs that were upregulated are shown in green to red, whereas the miRNAs that were downregulated are shown from red to green. (D, E) miR-92a-3p, miR-221-3p, miR-21, miR-146a, miR-196a, miR-383, miR-206 and miR-497 were further analyzed using qRT-PCR in SiHa/DDP and HeLa/DDP cells. Data are presented as means ± SD of three individual experiments. * p < 0.05, ** p < 0.01 vs . SiHa or HeLa cells.

Journal: Frontiers in Pharmacology

Article Title: MicroRNA-92a-3p Enhances Cisplatin Resistance by Regulating Krüppel-Like Factor 4-Mediated Cell Apoptosis and Epithelial-to-Mesenchymal Transition in Cervical Cancer

doi: 10.3389/fphar.2021.783213

Figure Lengend Snippet: The expression of miRNAs in cisplatin-sensitive and -resistant cervical cancer cells. Effects of cisplatin at different concentrations on cell viability were analyzed using CCK-8 assay in SiHa and SiHa/DDP (A) , and in HeLa and HeLa/DDP (B) . (C) Differentially expressed miRNAs were analyzed between cervical cancer tissues and the adjacent normal tissues group. Data were retrieved from Gene Expression Omnibus (GEO) dataset, with the accession number GSE19611. The color code in the heat map is linear and the expression levels of miRNAs that were upregulated are shown in green to red, whereas the miRNAs that were downregulated are shown from red to green. (D, E) miR-92a-3p, miR-221-3p, miR-21, miR-146a, miR-196a, miR-383, miR-206 and miR-497 were further analyzed using qRT-PCR in SiHa/DDP and HeLa/DDP cells. Data are presented as means ± SD of three individual experiments. * p < 0.05, ** p < 0.01 vs . SiHa or HeLa cells.

Article Snippet: Taqman miRNA assays (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A) were used to amplify the expression of miR-92a-3p (Assay ID: 477827_mir), miR-221-3p (Assay ID: 000524_mir), miR-21 (Assay ID: 477975_mir), miR-146a (Assay ID: 478399_mir), miR-196a (Assay ID: 478230_mir), miR-206 (Assay ID: 477968_mir), miR-383 (Assay ID: 478079_mir), miR-497 (Assay ID: 478138_mir ) and RNU6B (Assay ID: 001093 ) in cell lines and tissue samples.

Techniques: Expressing, CCK-8 Assay, Gene Expression, Quantitative RT-PCR